Detailed information of TA system TA104941

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1181833..1182527
• Replicon: chromosome
• Accession: NZ_CP029115
• Organism: Escherichia coli strain AR435

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: CSC22_RS06870
• Protein ID: WP_001094398.1
• Coordinates: 1182159..1182527 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CSC22_RS06865
• Protein ID: WP_077249034.1
• Coordinates: 1181833..1182138 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC22_RS06820	1177783..1178238	+	456	WP_000581506.1	hypothetical protein	-
CSC22_RS06825	1178339..1178547	+	209	Protein_1088	DUF905 domain-containing protein	-
CSC22_RS06835	1178648..1179469	+	822	WP_001234565.1	DUF945 domain-containing protein	-
CSC22_RS06845	1179811..1180284	+	474	WP_000855059.1	antirestriction protein	-
CSC22_RS06850	1180300..1180776	+	477	WP_001424026.1	RadC family protein	-
CSC22_RS06855	1180845..1181066	+	222	WP_000692345.1	DUF987 domain-containing protein	-
CSC22_RS06860	1181085..1181729	+	645	WP_000086755.1	hypothetical protein	-
CSC22_RS06865	1181833..1182138	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CSC22_RS06870	1182159..1182527	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
CSC22_RS06875	1182848..1183186	-	339	Protein_1096	LysR family transcriptional regulator	-
CSC22_RS06880	1183237..1184193	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
CSC22_RS06885	1184203..1186401	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
CSC22_RS06890	1186398..1187354	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1166174..1189072	22898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T104941 WP_001094398.1 NZ_CP029115:1182159-1182527 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T104941 NZ_CP029115:1182159-1182527 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT104941 WP_077249034.1 NZ_CP029115:1181833-1182138 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT104941 NZ_CP029115:1181833-1182138 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4