Detailed information of TA system TA104937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 624450..625045
• Replicon: chromosome
• Accession: NZ_CP029115
• Organism: Escherichia coli strain AR435

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: CSC22_RS03965
• Protein ID: WP_108703241.1
• Coordinates: 624695..625045 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: CSC22_RS03960
• Protein ID: WP_001223208.1
• Coordinates: 624450..624701 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC22_RS03950	620115..623894	+	3780	WP_000060978.1	autotransporter assembly complex protein TamB	-
CSC22_RS03955	623897..624238	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
CSC22_RS03960	624450..624701	+	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
CSC22_RS03965	624695..625045	+	351	WP_108703241.1	endoribonuclease toxin ChpB	Toxin
CSC22_RS03970	625125..625655	-	531	WP_000055072.1	inorganic diphosphatase	-
CSC22_RS03975	625965..626921	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
CSC22_RS03980	627061..628563	+	1503	WP_000205784.1	sugar ABC transporter ATP-binding protein	-
CSC22_RS03985	628577..629599	+	1023	WP_001295197.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12525.47 Da
• Isoelectric Point: 5.6219

>T104937 WP_108703241.1 NZ_CP029115:624695-625045 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGLVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T104937 NZ_CP029115:624695-625045 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCTTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT104937 WP_001223208.1 NZ_CP029115:624450-624701 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT104937 NZ_CP029115:624450-624701 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26