Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 99293..99818 Replicon plasmid unnamed1
Accession NZ_CP029113
Organism Escherichia coli strain AR435

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CSC22_RS00660 Protein ID WP_001159871.1
Coordinates 99513..99818 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CSC22_RS00655 Protein ID WP_000813630.1
Coordinates 99293..99511 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC22_RS00620 94503..95189 + 687 Protein_107 IS1 family transposase -
CSC22_RS00630 95443..96465 - 1023 WP_000361402.1 helicase UvrD -
CSC22_RS00635 96450..98015 - 1566 WP_001128474.1 AAA family ATPase -
CSC22_RS00640 98090..98506 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
CSC22_RS00645 98503..98733 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CSC22_RS00655 99293..99511 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CSC22_RS00660 99513..99818 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CSC22_RS00665 99819..100625 + 807 WP_000016970.1 site-specific integrase -
CSC22_RS00670 101347..102102 + 756 WP_000852146.1 replication initiation protein RepE -
CSC22_RS00675 102690..103856 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aadA5 / qacE / sul1 / mph(A) - 1..114412 114412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T104935 WP_001159871.1 NZ_CP029113:99513-99818 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T104935 NZ_CP029113:99513-99818 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT104935 WP_000813630.1 NZ_CP029113:99293-99511 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT104935 NZ_CP029113:99293-99511 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References