Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 42671..43296 Replicon plasmid unnamed1
Accession NZ_CP029113
Organism Escherichia coli strain AR435

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D7QA53
Locus tag CSC22_RS00280 Protein ID WP_039023147.1
Coordinates 42671..43069 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D7QA08
Locus tag CSC22_RS00285 Protein ID WP_039023146.1
Coordinates 43069..43296 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC22_RS00265 38915..39412 + 498 WP_000605862.1 entry exclusion protein -
CSC22_RS00270 39444..40175 + 732 WP_000850422.1 complement resistance protein TraT -
CSC22_RS00275 40428..42662 + 2235 WP_062914766.1 type IV conjugative transfer system coupling protein TraD -
CSC22_RS00280 42671..43069 - 399 WP_039023147.1 tRNA(fMet)-specific endonuclease VapC Toxin
CSC22_RS00285 43069..43296 - 228 WP_039023146.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aadA5 / qacE / sul1 / mph(A) - 1..114412 114412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14885.26 Da        Isoelectric Point: 8.2824

>T104933 WP_039023147.1 NZ_CP029113:c43069-42671 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARCRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T104933 NZ_CP029113:c43069-42671 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTTAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCATACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCTG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8549.62 Da        Isoelectric Point: 4.7513

>AT104933 WP_039023146.1 NZ_CP029113:c43296-43069 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAIGRTRLITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT104933 NZ_CP029113:c43296-43069 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTATCGGACGAACCCGACTCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAACGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7QA53

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7QA08

References