Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3338860..3339491 Replicon chromosome
Accession NZ_CP029111
Organism Escherichia coli strain AR436

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag CSC23_RS17695 Protein ID WP_001259388.1
Coordinates 3338860..3339135 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CSC23_RS17700 Protein ID WP_000593555.1
Coordinates 3339132..3339491 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC23_RS17680 3333864..3334931 + 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -
CSC23_RS17685 3334928..3337663 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
CSC23_RS17690 3337663..3338787 + 1125 WP_001615305.1 ABC transporter permease -
CSC23_RS17695 3338860..3339135 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
CSC23_RS17700 3339132..3339491 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CSC23_RS17705 3339611..3340012 - 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
CSC23_RS17710 3340018..3340824 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
CSC23_RS17715 3340821..3341585 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
CSC23_RS17720 3341585..3342418 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CSC23_RS17725 3342415..3343359 - 945 WP_094322784.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T104923 WP_001259388.1 NZ_CP029111:3338860-3339135 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T104923 NZ_CP029111:3338860-3339135 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT104923 WP_000593555.1 NZ_CP029111:3339132-3339491 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT104923 NZ_CP029111:3339132-3339491 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References