Detailed information of TA system TA104917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1732488..1733325
• Replicon: chromosome
• Accession: NZ_CP029111
• Organism: Escherichia coli strain AR436

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: CSC23_RS09610
• Protein ID: WP_000227788.1
• Coordinates: 1732783..1733325 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: CSC23_RS09605
• Protein ID: WP_001297137.1
• Coordinates: 1732488..1732799 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC23_RS09580	1727508..1728455	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
CSC23_RS09585	1728477..1730468	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CSC23_RS09590	1730458..1731072	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CSC23_RS09595	1731072..1731401	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CSC23_RS09600	1731413..1732303	+	891	WP_000971332.1	heme o synthase	-
CSC23_RS09605	1732488..1732799	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
CSC23_RS09610	1732783..1733325	+	543	WP_000227788.1	GNAT family N-acetyltransferase	Toxin
CSC23_RS09615	1733381..1734316	-	936	WP_001297127.1	sel1 repeat family protein	-
CSC23_RS09620	1734724..1736088	+	1365	WP_001000978.1	MFS transporter	-
CSC23_RS09625	1736216..1736707	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
CSC23_RS09630	1736875..1737786	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (79-160)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19835.05 Da
• Isoelectric Point: 8.3395

>T104917 WP_000227788.1 NZ_CP029111:1732783-1733325 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFSQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T104917 NZ_CP029111:1732783-1733325 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTAGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT104917 WP_001297137.1 NZ_CP029111:1732488-1732799 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT104917 NZ_CP029111:1732488-1732799 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
TGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3