Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 75062..75587 Replicon plasmid unnamed2
Accession NZ_CP029110
Organism Escherichia coli strain AR436

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CSC23_RS00480 Protein ID WP_001159871.1
Coordinates 75282..75587 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CSC23_RS00475 Protein ID WP_000813630.1
Coordinates 75062..75280 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC23_RS00440 70063..70653 - 591 WP_000194550.1 hypothetical protein -
CSC23_RS00445 70653..70907 - 255 WP_000343103.1 hypothetical protein -
CSC23_RS00450 71259..72260 + 1002 WP_000774875.1 DUF4238 domain-containing protein -
CSC23_RS00455 72436..72780 - 345 WP_000793307.1 hypothetical protein -
CSC23_RS00460 73279..73533 - 255 WP_000678527.1 hypothetical protein -
CSC23_RS00465 73578..74504 - 927 WP_001290416.1 hypothetical protein -
CSC23_RS00475 75062..75280 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CSC23_RS00480 75282..75587 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CSC23_RS00485 75588..76394 + 807 WP_000016969.1 site-specific integrase -
CSC23_RS00490 76574..77218 + 645 WP_001144036.1 ParA family protein -
CSC23_RS00495 77305..77613 + 309 WP_000030199.1 hypothetical protein -
CSC23_RS00500 78027..79007 + 981 WP_000688514.1 plasmid segregation protein ParM -
CSC23_RS00505 79000..79416 + 417 WP_001278818.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCMY-2 - 1..91246 91246


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T104905 WP_001159871.1 NZ_CP029110:75282-75587 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T104905 NZ_CP029110:75282-75587 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT104905 WP_000813630.1 NZ_CP029110:75062-75280 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT104905 NZ_CP029110:75062-75280 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References