Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 39870..40471 Replicon plasmid unnamed1
Accession NZ_CP029103
Organism Escherichia coli strain AR437

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag CSC24_RS00275 Protein ID WP_001216045.1
Coordinates 40091..40471 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CSC24_RS00270 Protein ID WP_001190712.1
Coordinates 39870..40091 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC24_RS26230 34965..35144 + 180 Protein_36 hypothetical protein -
CSC24_RS00245 35243..35989 - 747 Protein_37 IS1 family transposase -
CSC24_RS00250 36215..38071 + 1857 WP_077902027.1 acyltransferase -
CSC24_RS00255 38642..39016 - 375 Protein_39 integrase core domain-containing protein -
CSC24_RS00260 39018..39233 - 216 Protein_40 DNA-binding protein -
CSC24_RS00265 39408..39797 + 390 WP_000506730.1 DNA repair protein -
CSC24_RS00270 39870..40091 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CSC24_RS00275 40091..40471 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CSC24_RS00280 40476..40655 + 180 WP_001339207.1 hypothetical protein -
CSC24_RS00285 40683..41726 + 1044 WP_023356283.1 DUF968 domain-containing protein -
CSC24_RS00290 41815..42267 + 453 WP_001326849.1 late promoter-activating protein -
CSC24_RS00295 42353..43546 + 1194 WP_000219625.1 hypothetical protein -
CSC24_RS00300 43546..45030 + 1485 WP_000124150.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..89643 89643
- flank IS/Tn - - 38642..39016 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T104873 WP_001216045.1 NZ_CP029103:40091-40471 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T104873 NZ_CP029103:40091-40471 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT104873 WP_001190712.1 NZ_CP029103:39870-40091 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT104873 NZ_CP029103:39870-40091 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References