Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 43962..44605 Replicon plasmid unnamed4
Accession NZ_CP029102
Organism Klebsiella pneumoniae strain AR438

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CSC25_RS29610 Protein ID WP_001044770.1
Coordinates 44189..44605 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CSC25_RS29605 Protein ID WP_001261282.1
Coordinates 43962..44192 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC25_RS29560 39160..39633 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
CSC25_RS29565 39725..39955 + 231 WP_011977773.1 hypothetical protein -
CSC25_RS29570 40847..41629 - 783 WP_004152340.1 site-specific integrase -
CSC25_RS29575 41629..41961 - 333 WP_004152339.1 hypothetical protein -
CSC25_RS29580 41968..42366 - 399 WP_004171440.1 hypothetical protein -
CSC25_RS29585 42392..42721 - 330 WP_004152337.1 hypothetical protein -
CSC25_RS29590 42749..43057 - 309 WP_004152336.1 hypothetical protein -
CSC25_RS29600 43544..44005 - 462 WP_072093212.1 hypothetical protein -
CSC25_RS29605 43962..44192 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CSC25_RS29610 44189..44605 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CSC25_RS29615 44679..45389 + 711 WP_004152334.1 ATP-binding protein -
CSC25_RS29620 45423..46120 + 698 Protein_57 IS1 family transposase -
CSC25_RS29625 46136..46246 + 111 Protein_58 mercuric transport protein periplasmic component -
CSC25_RS29630 46282..46704 + 423 WP_001340589.1 organomercurial transporter MerC -
CSC25_RS29635 46756..48450 + 1695 WP_000105636.1 mercury(II) reductase -
CSC25_RS29640 48468..48830 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
CSC25_RS29645 48827..49063 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1A / blaOXA-9 / blaKPC-3 - 1..135655 135655
- flank IS/Tn - - 37772..39040 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T104872 WP_001044770.1 NZ_CP029102:44189-44605 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T104872 NZ_CP029102:44189-44605 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT104872 WP_001261282.1 NZ_CP029102:43962-44192 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT104872 NZ_CP029102:43962-44192 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References