Detailed information of TA system TA104850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4426315..4427357
• Replicon: chromosome
• Accession: NZ_CP029097
• Organism: Pseudomonas aeruginosa strain AR439

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CSC26_RS23525
• Protein ID: WP_003153636.1
• Coordinates: 4426315..4426890 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: CSC26_RS23530
• Protein ID: WP_003050245.1
• Coordinates: 4426887..4427357 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC26_RS23500	4422753..4423478	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
CSC26_RS23505	4423517..4424419	-	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
CSC26_RS23510	4424419..4424919	-	501	WP_003090159.1	hypothetical protein	-
CSC26_RS23515	4424916..4425386	-	471	WP_003090160.1	hypothetical protein	-
CSC26_RS23520	4425383..4426297	-	915	WP_016852809.1	AAA family ATPase	-
CSC26_RS23525	4426315..4426890	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
CSC26_RS23530	4426887..4427357	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
CSC26_RS23535	4427561..4427944	+	384	WP_108720437.1	RAQPRD family integrative conjugative element protein	-
CSC26_RS23540	4427941..4428174	+	234	WP_006226027.1	TIGR03758 family integrating conjugative element protein	-
CSC26_RS23545	4428191..4428550	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
CSC26_RS23550	4428562..4428960	+	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
CSC26_RS23555	4428957..4429649	+	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
CSC26_RS23560	4429646..4430557	+	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
CSC26_RS23565	4430547..4431965	+	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4380199..4501932	121733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T104850 WP_003153636.1 NZ_CP029097:c4426890-4426315 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T104850 NZ_CP029097:c4426890-4426315 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT104850 WP_003050245.1 NZ_CP029097:c4427357-4426887 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT104850 NZ_CP029097:c4427357-4426887 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68