Detailed information of TA system TA104840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 6382272..6382777
• Replicon: chromosome
• Accession: NZ_CP029090
• Organism: Pseudomonas aeruginosa strain AR442

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: CSC29_RS31170
• Protein ID: WP_003083773.1
• Coordinates: 6382272..6382553 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: CSC29_RS31175
• Protein ID: WP_003083775.1
• Coordinates: 6382550..6382777 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC29_RS31145	6377523..6378872	+	1350	WP_023464185.1	C4-dicarboxylate transporter DctA	-
CSC29_RS31150	6378921..6379607	+	687	WP_003083762.1	FadR family transcriptional regulator	-
CSC29_RS31155	6379708..6380442	+	735	WP_003117208.1	GntR family transcriptional regulator	-
CSC29_RS31160	6380646..6381032	+	387	WP_003083767.1	aegerolysin family protein	-
CSC29_RS31165	6381064..6381972	-	909	WP_003083769.1	LysR family transcriptional regulator	-
CSC29_RS31170	6382272..6382553	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
CSC29_RS31175	6382550..6382777	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
CSC29_RS31180	6382953..6383573	-	621	WP_031298866.1	hypothetical protein	-
CSC29_RS31185	6383674..6384174	+	501	WP_003101228.1	LEA type 2 family protein	-
CSC29_RS31190	6384247..6384588	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
CSC29_RS31195	6384670..6386097	-	1428	WP_023464187.1	GABA permease	-
CSC29_RS31200	6386266..6387759	-	1494	WP_016263977.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T104840 WP_003083773.1 NZ_CP029090:c6382553-6382272 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T104840 NZ_CP029090:c6382553-6382272 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT104840 WP_003083775.1 NZ_CP029090:c6382777-6382550 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT104840 NZ_CP029090:c6382777-6382550 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9