Detailed information of TA system TA104838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4918571..4919157
• Replicon: chromosome
• Accession: NZ_CP029090
• Organism: Pseudomonas aeruginosa strain AR442

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: CSC29_RS24145
• Protein ID: WP_003120987.1
• Coordinates: 4918858..4919157 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CSC29_RS24140
• Protein ID: WP_003448662.1
• Coordinates: 4918571..4918861 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC29_RS24120	4913722..4913931	+	210	WP_003105733.1	cold-shock protein	-
CSC29_RS24125	4914153..4916042	+	1890	WP_016851610.1	hypothetical protein	-
CSC29_RS24130	4916039..4918015	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
CSC29_RS24135	4918156..4918500	+	345	WP_016851612.1	hypothetical protein	-
CSC29_RS24140	4918571..4918861	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
CSC29_RS24145	4918858..4919157	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC29_RS24150	4919359..4920483	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
CSC29_RS24155	4920483..4922192	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
CSC29_RS24160	4922196..4923521	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
CSC29_RS24165	4923511..4924044	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4891592..4986069	94477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T104838 WP_003120987.1 NZ_CP029090:c4919157-4918858 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T104838 NZ_CP029090:c4919157-4918858 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT104838 WP_003448662.1 NZ_CP029090:c4918861-4918571 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT104838 NZ_CP029090:c4918861-4918571 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73