Detailed information of TA system TA104833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6751618..6752204
• Replicon: chromosome
• Accession: NZ_CP029089
• Organism: Pseudomonas aeruginosa strain AR444

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: CSC31_RS32045
• Protein ID: WP_003120987.1
• Coordinates: 6751618..6751917 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CSC31_RS32050
• Protein ID: WP_003448662.1
• Coordinates: 6751914..6752204 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC31_RS32025	6746731..6747264	-	534	WP_003149521.1	type IV pilus biogenesis protein PilP	-
CSC31_RS32030	6747254..6748579	-	1326	WP_022579625.1	type 4b pilus protein PilO2	-
CSC31_RS32035	6748583..6750292	-	1710	WP_003123331.1	PilN family type IVB pilus formation outer membrane protein	-
CSC31_RS32040	6750292..6751416	-	1125	WP_024007699.1	TcpQ domain-containing protein	-
CSC31_RS32045	6751618..6751917	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC31_RS32050	6751914..6752204	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
CSC31_RS32055	6752287..6752622	-	336	WP_022579623.1	hypothetical protein	-
CSC31_RS32060	6752761..6754737	-	1977	WP_023093671.1	DEAD/DEAH box helicase	-
CSC31_RS32065	6754734..6756623	-	1890	WP_023093670.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	6693279..6782711	89432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T104833 WP_003120987.1 NZ_CP029089:6751618-6751917 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T104833 NZ_CP029089:6751618-6751917 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT104833 WP_003448662.1 NZ_CP029089:6751914-6752204 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT104833 NZ_CP029089:6751914-6752204 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73