Detailed information of TA system TA104831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 5333748..5334253
• Replicon: chromosome
• Accession: NZ_CP029089
• Organism: Pseudomonas aeruginosa strain AR444

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: CSC31_RS25150
• Protein ID: WP_003083773.1
• Coordinates: 5333972..5334253 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: CSC31_RS25145
• Protein ID: WP_003083775.1
• Coordinates: 5333748..5333975 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC31_RS25120	5328767..5330260	+	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
CSC31_RS25125	5330429..5331856	+	1428	WP_003083784.1	GABA permease	-
CSC31_RS25130	5331938..5332279	-	342	WP_003101229.1	alkylphosphonate utilization protein	-
CSC31_RS25135	5332352..5332852	-	501	WP_003162763.1	LEA type 2 family protein	-
CSC31_RS25140	5332953..5333573	+	621	WP_003101226.1	hypothetical protein	-
CSC31_RS25145	5333748..5333975	+	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
CSC31_RS25150	5333972..5334253	+	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
CSC31_RS25155	5334553..5335461	+	909	WP_003083769.1	LysR family transcriptional regulator	-
CSC31_RS25160	5335493..5335879	-	387	WP_003083767.1	aegerolysin family protein	-
CSC31_RS25165	5336083..5336817	-	735	WP_003117208.1	GntR family transcriptional regulator	-
CSC31_RS25170	5336918..5337604	-	687	WP_003083762.1	FadR family transcriptional regulator	-
CSC31_RS25175	5337653..5339002	-	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T104831 WP_003083773.1 NZ_CP029089:5333972-5334253 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T104831 NZ_CP029089:5333972-5334253 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT104831 WP_003083775.1 NZ_CP029089:5333748-5333975 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT104831 NZ_CP029089:5333748-5333975 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9