Detailed information of TA system TA104822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2933160..2934202
• Replicon: chromosome
• Accession: NZ_CP029088
• Organism: Pseudomonas aeruginosa strain AR445

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CSC32_RS14470
• Protein ID: WP_003153636.1
• Coordinates: 2933627..2934202 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: CSC32_RS14465
• Protein ID: WP_003050245.1
• Coordinates: 2933160..2933630 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC32_RS14430	2928552..2929970	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
CSC32_RS14435	2929960..2930871	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
CSC32_RS14440	2930868..2931560	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
CSC32_RS14445	2931557..2931955	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
CSC32_RS14450	2931967..2932326	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
CSC32_RS14455	2932343..2932576	-	234	WP_023101201.1	TIGR03758 family integrating conjugative element protein	-
CSC32_RS14460	2932573..2932956	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
CSC32_RS14465	2933160..2933630	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
CSC32_RS14470	2933627..2934202	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
CSC32_RS14475	2934220..2935134	+	915	WP_016852809.1	AAA family ATPase	-
CSC32_RS14480	2935131..2935601	+	471	WP_003090160.1	hypothetical protein	-
CSC32_RS14485	2935598..2936098	+	501	WP_003090159.1	hypothetical protein	-
CSC32_RS14490	2936098..2937000	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
CSC32_RS14495	2937039..2937764	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T104822 WP_003153636.1 NZ_CP029088:2933627-2934202 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T104822 NZ_CP029088:2933627-2934202 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT104822 WP_003050245.1 NZ_CP029088:2933160-2933630 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT104822 NZ_CP029088:2933160-2933630 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68