Detailed information of TA system TA104810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2229292..2229821
• Replicon: chromosome
• Accession: NZ_CP029087
• Organism: Staphylococcus aureus strain AR461

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CSC47_RS11765
• Protein ID: WP_000621175.1
• Coordinates: 2229292..2229654 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CSC47_RS11770
• Protein ID: WP_000948331.1
• Coordinates: 2229651..2229821 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC47_RS11740	2226270..2227040	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
CSC47_RS11745	2227015..2227494	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
CSC47_RS11750	2227496..2227822	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CSC47_RS11755	2227941..2228942	-	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
CSC47_RS11765	2229292..2229654	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSC47_RS11770	2229651..2229821	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CSC47_RS11775	2229906..2231054	-	1149	WP_001281154.1	alanine racemase	-
CSC47_RS11780	2231120..2231479	-	360	WP_000581197.1	holo-ACP synthase	-
CSC47_RS11785	2231483..2231974	-	492	WP_001205912.1	PH domain-containing protein	-
CSC47_RS11790	2231961..2233544	-	1584	WP_001294626.1	PH domain-containing protein	-
CSC47_RS11795	2233537..2234016	-	480	WP_001287087.1	hypothetical protein	-
CSC47_RS11800	2234224..2234784	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T104810 WP_000621175.1 NZ_CP029087:c2229654-2229292 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T104810 NZ_CP029087:c2229654-2229292 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT104810 WP_000948331.1 NZ_CP029087:c2229821-2229651 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT104810 NZ_CP029087:c2229821-2229651 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1