Detailed information of TA system TA104776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 24455..24984
• Replicon: chromosome
• Accession: NZ_CP029084
• Organism: Staphylococcus aureus strain AR464

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CSC49_RS00260
• Protein ID: WP_000621175.1
• Coordinates: 24455..24817 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CSC49_RS00265
• Protein ID: WP_000948331.1
• Coordinates: 24814..24984 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC49_RS00235	21434..22204	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
CSC49_RS00240	22179..22658	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
CSC49_RS00245	22660..22986	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CSC49_RS00250	23105..24106	-	1002	WP_042744356.1	PP2C family protein-serine/threonine phosphatase	-
CSC49_RS00260	24455..24817	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSC49_RS00265	24814..24984	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CSC49_RS00270	25069..26217	-	1149	WP_001281140.1	alanine racemase	-
CSC49_RS00275	26283..26642	-	360	WP_000581199.1	holo-ACP synthase	-
CSC49_RS00280	26646..27137	-	492	WP_001205907.1	PH domain-containing protein	-
CSC49_RS00285	27124..28707	-	1584	WP_001294637.1	PH domain-containing protein	-
CSC49_RS00290	28700..29179	-	480	WP_001287077.1	hypothetical protein	-
CSC49_RS00295	29387..29947	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T104776 WP_000621175.1 NZ_CP029084:c24817-24455 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T104776 NZ_CP029084:c24817-24455 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT104776 WP_000948331.1 NZ_CP029084:c24984-24814 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT104776 NZ_CP029084:c24984-24814 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1