Detailed information of TA system TA104766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1611722..1612251
• Replicon: chromosome
• Accession: NZ_CP029082
• Organism: Staphylococcus aureus strain AR465

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CSC50_RS08530
• Protein ID: WP_000621175.1
• Coordinates: 1611889..1612251 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CSC50_RS08525
• Protein ID: WP_000948331.1
• Coordinates: 1611722..1611892 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC50_RS08495	1606759..1607319	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
CSC50_RS08500	1607527..1608006	+	480	WP_001287087.1	hypothetical protein	-
CSC50_RS08505	1607999..1609582	+	1584	WP_001294626.1	PH domain-containing protein	-
CSC50_RS08510	1609569..1610060	+	492	WP_001205912.1	PH domain-containing protein	-
CSC50_RS08515	1610064..1610423	+	360	WP_000581197.1	holo-ACP synthase	-
CSC50_RS08520	1610489..1611637	+	1149	WP_001281154.1	alanine racemase	-
CSC50_RS08525	1611722..1611892	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CSC50_RS08530	1611889..1612251	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSC50_RS08540	1612601..1613602	+	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
CSC50_RS08545	1613721..1614047	+	327	WP_001052491.1	anti-sigma factor antagonist	-
CSC50_RS08550	1614049..1614528	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
CSC50_RS08555	1614503..1615273	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T104766 WP_000621175.1 NZ_CP029082:1611889-1612251 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T104766 NZ_CP029082:1611889-1612251 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT104766 WP_000948331.1 NZ_CP029082:1611722-1611892 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT104766 NZ_CP029082:1611722-1611892 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1