Detailed information of TA system TA104747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1257103..1258020
• Replicon: chromosome
• Accession: NZ_CP029071
• Organism: Bacillus amyloliquefaciens strain ALB79

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: DDT10_RS06520
• Protein ID: WP_007407256.1
• Coordinates: 1257274..1258020 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: DDT10_RS06515
• Protein ID: WP_003154807.1
• Coordinates: 1257103..1257273 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDT10_RS06480	1252314..1253903	+	1590	WP_053573489.1	hypothetical protein	-
DDT10_RS06485	1253916..1254341	+	426	WP_053573490.1	hypothetical protein	-
DDT10_RS06490	1254346..1254543	+	198	WP_012117366.1	XkdX family protein	-
DDT10_RS06495	1254600..1255361	+	762	WP_053573491.1	hypothetical protein	-
DDT10_RS06500	1255413..1255676	+	264	WP_003154815.1	hemolysin XhlA family protein	-
DDT10_RS06505	1255690..1255953	+	264	WP_003154813.1	phage holin	-
DDT10_RS06510	1255967..1256845	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
DDT10_RS06515	1257103..1257273	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
DDT10_RS06520	1257274..1258020	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
DDT10_RS06525	1258124..1259122	-	999	WP_003154805.1	inorganic phosphate transporter	-
DDT10_RS06530	1259135..1259752	-	618	WP_003154804.1	DUF47 domain-containing protein	-
DDT10_RS06535	1260038..1261354	-	1317	WP_007610842.1	amino acid permease	-
DDT10_RS06540	1261677..1262627	+	951	WP_031378808.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1222572..1294062	71490

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T104747 WP_007407256.1 NZ_CP029071:c1258020-1257274 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T104747 NZ_CP029071:c1258020-1257274 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT104747 WP_003154807.1 NZ_CP029071:c1257273-1257103 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT104747 NZ_CP029071:c1257273-1257103 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14