Detailed information of TA system TA104746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 521657..522294
• Replicon: chromosome
• Accession: NZ_CP029071
• Organism: Bacillus amyloliquefaciens strain ALB79

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: DDT10_RS02525
• Protein ID: WP_003156187.1
• Coordinates: 521944..522294 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: DDT10_RS02520
• Protein ID: WP_003156188.1
• Coordinates: 521657..521938 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDT10_RS02500	518022..518621	-	600	WP_053573675.1	rhomboid family intramembrane serine protease	-
DDT10_RS02505	518714..519079	+	366	WP_015416872.1	holo-ACP synthase	-
DDT10_RS02510	519244..520251	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
DDT10_RS02515	520368..521537	+	1170	WP_053573676.1	alanine racemase	-
DDT10_RS02520	521657..521938	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
DDT10_RS02525	521944..522294	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
DDT10_RS02530	522412..523233	+	822	WP_033575045.1	STAS domain-containing protein	-
DDT10_RS02535	523238..523603	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
DDT10_RS02540	523606..524007	+	402	WP_003156178.1	anti-sigma regulatory factor	-
DDT10_RS02545	524019..525026	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
DDT10_RS02550	525090..525419	+	330	WP_033575044.1	anti-sigma factor antagonist	-
DDT10_RS02555	525416..525898	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
DDT10_RS02560	525864..526652	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
DDT10_RS02565	526652..527254	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T104746 WP_003156187.1 NZ_CP029071:521944-522294 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T104746 NZ_CP029071:521944-522294 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT104746 WP_003156188.1 NZ_CP029071:521657-521938 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT104746 NZ_CP029071:521657-521938 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5