Detailed information of TA system TA104745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1311154..1312071
• Replicon: chromosome
• Accession: NZ_CP029070
• Organism: Bacillus amyloliquefaciens strain ALB69

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: DDT09_RS06960
• Protein ID: WP_007407256.1
• Coordinates: 1311325..1312071 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: DDT09_RS06955
• Protein ID: WP_014417527.1
• Coordinates: 1311154..1311324 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDT09_RS06920	1306387..1308009	+	1623	WP_061862105.1	pyocin knob domain-containing protein	-
DDT09_RS06925	1308022..1308393	+	372	WP_061862106.1	hypothetical protein	-
DDT09_RS06930	1308398..1308595	+	198	WP_007610833.1	XkdX family protein	-
DDT09_RS06935	1308652..1309413	+	762	WP_014417526.1	hypothetical protein	-
DDT09_RS06940	1309465..1309728	+	264	WP_003154815.1	hemolysin XhlA family protein	-
DDT09_RS06945	1309742..1310005	+	264	WP_003154813.1	phage holin	-
DDT09_RS06950	1310019..1310897	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
DDT09_RS06955	1311154..1311324	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
DDT09_RS06960	1311325..1312071	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
DDT09_RS06965	1312175..1313173	-	999	WP_003154805.1	inorganic phosphate transporter	-
DDT09_RS06970	1313186..1313803	-	618	WP_003154804.1	DUF47 domain-containing protein	-
DDT09_RS06975	1314089..1315405	-	1317	WP_061862107.1	amino acid permease	-
DDT09_RS06980	1315728..1316678	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1277524..1370201	92677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T104745 WP_007407256.1 NZ_CP029070:c1312071-1311325 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T104745 NZ_CP029070:c1312071-1311325 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT104745 WP_014417527.1 NZ_CP029070:c1311324-1311154 [Bacillus amyloliquefaciens]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT104745 NZ_CP029070:c1311324-1311154 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8