Detailed information of TA system TA104742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 533245..533882
• Replicon: chromosome
• Accession: NZ_CP029069
• Organism: Bacillus amyloliquefaciens strain ALB65

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BAALB65_RS02840
• Protein ID: WP_003156187.1
• Coordinates: 533532..533882 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BAALB65_RS02835
• Protein ID: WP_003156188.1
• Coordinates: 533245..533526 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAALB65_RS02815	529610..530209	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
BAALB65_RS02820	530302..530667	+	366	WP_014304402.1	holo-ACP synthase	-
BAALB65_RS02825	530832..531839	+	1008	WP_109566717.1	outer membrane lipoprotein carrier protein LolA	-
BAALB65_RS02830	531956..533125	+	1170	WP_014416966.1	alanine racemase	-
BAALB65_RS02835	533245..533526	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BAALB65_RS02840	533532..533882	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAALB65_RS02845	534000..534821	+	822	WP_003156182.1	STAS domain-containing protein	-
BAALB65_RS02850	534826..535191	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
BAALB65_RS02855	535194..535595	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BAALB65_RS02860	535607..536614	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
BAALB65_RS02865	536678..537007	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BAALB65_RS02870	537004..537486	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
BAALB65_RS02875	537452..538240	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BAALB65_RS02880	538240..538842	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T104742 WP_003156187.1 NZ_CP029069:533532-533882 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T104742 NZ_CP029069:533532-533882 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT104742 WP_003156188.1 NZ_CP029069:533245-533526 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT104742 NZ_CP029069:533245-533526 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5