Detailed information of TA system TA104741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 51935..52506
• Replicon: plasmid pPe10302
• Accession: NZ_CP029068
• Organism: Enterococcus mundtii strain Pe103

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DDJ96_RS15320
• Protein ID: WP_137073140.1
• Coordinates: 52165..52506 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: V5XUJ2
• Locus tag: DDJ96_RS15315
• Protein ID: WP_023520685.1
• Coordinates: 51935..52165 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDJ96_RS15285	48021..48371	+	351	WP_137073135.1	hypothetical protein	-
DDJ96_RS15290	48751..49491	+	741	WP_137073136.1	trypsin-like peptidase domain-containing protein	-
DDJ96_RS15295	49759..49959	+	201	WP_086335682.1	cold-shock protein	-
DDJ96_RS15300	50026..50241	+	216	WP_137073137.1	DUF2187 family protein	-
DDJ96_RS15305	50350..50985	-	636	WP_137073138.1	recombinase family protein	-
DDJ96_RS15310	51114..51695	-	582	WP_137073139.1	recombinase family protein	-
DDJ96_RS15315	51935..52165	+	231	WP_023520685.1	hypothetical protein	Antitoxin
DDJ96_RS15320	52165..52506	+	342	WP_137073140.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DDJ96_RS15325	52758..52988	+	231	WP_074802108.1	hypothetical protein	-
DDJ96_RS15330	53048..54301	+	1254	WP_137072012.1	efflux RND transporter periplasmic adaptor subunit	-
DDJ96_RS15335	54288..54971	+	684	WP_137072014.1	ABC transporter ATP-binding protein	-
DDJ96_RS15340	54978..56210	+	1233	WP_137072016.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56223	56223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13088.31 Da
• Isoelectric Point: 10.1552

>T104741 WP_137073140.1 NZ_CP029068:52165-52506 [Enterococcus mundtii]
VLEDKNYIPKKGDIVWIDFDPSTGNEIQKRRPGLVVSRHEFNRKTMFAVICPITSTIKNLPTRYSLPADLDTNGQVLISQ
LKSLDFKKRKLKKVENLPLQDMAKIDQIIQYIF

Nucleotide

• Length: 342 bp

>T104741 NZ_CP029068:52165-52506 [Enterococcus mundtii]
GTGTTAGAAGATAAAAATTACATTCCTAAAAAAGGAGACATTGTTTGGATTGATTTTGATCCTTCAACAGGAAACGAAAT
TCAAAAGAGACGTCCAGGATTAGTTGTTTCTCGACATGAGTTTAATCGGAAGACAATGTTTGCGGTAATCTGTCCAATTA
CTTCTACTATAAAGAATCTACCGACGCGCTATTCTCTTCCTGCAGATTTAGATACAAATGGGCAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTCAAGAAAAGAAAACTCAAAAAGGTAGAAAATTTACCTTTACAAGATATGGCTAAAATTGATCA
GATTATCCAGTATATATTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.8852

>AT104741 WP_023520685.1 NZ_CP029068:51935-52165 [Enterococcus mundtii]
MLITKSRLQGSSVVITLPSDNGKKPSDNQEYIVVYSDDGTITLVPKIDDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT104741 NZ_CP029068:51935-52165 [Enterococcus mundtii]
ATGCTTATTACAAAATCTAGATTACAAGGAAGTTCTGTAGTAATTACTCTTCCGTCTGATAATGGTAAAAAACCTTCAGA
TAACCAAGAATATATCGTTGTATATTCTGATGATGGAACAATTACGTTAGTTCCTAAAATCGATGATCCATTTAGTGGAG
GAGAAGAAGCTGAGTATTATGAAAAAGATGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• AlphaFold DB: V5XUJ2