Detailed information of TA system TA104740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 42362..42971
• Replicon: plasmid pPe10301
• Accession: NZ_CP029067
• Organism: Enterococcus mundtii strain Pe103

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: DDJ96_RS14960
• Protein ID: WP_023520664.1
• Coordinates: 42362..42550 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A022N4U5
• Locus tag: DDJ96_RS14965
• Protein ID: WP_034690421.1
• Coordinates: 42585..42971 (+)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDJ96_RS14940	38229..39902	+	1674	WP_137073108.1	hypothetical protein	-
DDJ96_RS14945	39886..40167	+	282	WP_126133781.1	hypothetical protein	-
DDJ96_RS14950	40406..40792	+	387	WP_126133780.1	plasmid mobilization relaxosome protein MobC	-
DDJ96_RS14955	40794..42296	+	1503	WP_126133779.1	relaxase/mobilization nuclease domain-containing protein	-
DDJ96_RS14960	42362..42550	+	189	WP_023520664.1	type II toxin-antitoxin system HicA family toxin	Toxin
DDJ96_RS14965	42585..42971	+	387	WP_034690421.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DDJ96_RS14970	42991..43773	+	783	WP_137073109.1	Fic family protein	-
DDJ96_RS14975	43875..44132	+	258	WP_137073110.1	hypothetical protein	-
DDJ96_RS14985	44834..45373	-	540	WP_126133778.1	hypothetical protein	-
DDJ96_RS14990	45853..46194	+	342	WP_137073111.1	LPXTG cell wall anchor domain-containing protein	-
DDJ96_RS14995	46205..47068	+	864	WP_126133777.1	WxL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63713	63713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-100)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7019.30 Da
• Isoelectric Point: 11.3094

>T104740 WP_023520664.1 NZ_CP029067:42362-42550 [Enterococcus mundtii]
MPMTIREAEKLLKDAGFVEVKGGKGSHRKFIKKNFPRPVILTSHSKELSRRVEDSVRKAVGK

Nucleotide

• Length: 189 bp

>T104740 NZ_CP029067:42362-42550 [Enterococcus mundtii]
ATGCCCATGACAATTAGAGAAGCAGAGAAATTACTCAAAGATGCTGGCTTTGTTGAAGTGAAGGGTGGAAAGGGGTCACA
CAGGAAATTTATAAAGAAAAATTTTCCTAGACCAGTTATTCTTACCAGTCATTCAAAAGAGTTATCTAGAAGAGTAGAGG
ATTCTGTTCGAAAAGCAGTGGGCAAATAA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14050.16 Da
• Isoelectric Point: 4.3217

>AT104740 WP_034690421.1 NZ_CP029067:42585-42971 [Enterococcus mundtii]
MKKVYPAIFEKDPVGYGIYFPDVEGAVTQSEDIAQGLELAADALGIMLGDLVEKNMPLPTPTAINALSVNPETEFATLIS
VDLNDYLKDVQLDKKTIKIPHWLNVRATNEGINFSKTMTEALAQKLNV

Nucleotide

• Length: 387 bp

>AT104740 NZ_CP029067:42585-42971 [Enterococcus mundtii]
ATGAAAAAAGTTTATCCAGCTATATTTGAAAAAGATCCTGTTGGATACGGAATTTATTTTCCTGATGTTGAAGGAGCGGT
CACGCAATCGGAAGATATCGCCCAAGGATTAGAACTTGCGGCAGATGCGTTAGGTATTATGCTTGGAGACCTAGTTGAAA
AAAACATGCCTTTGCCAACACCTACGGCTATCAATGCCCTTTCTGTAAACCCTGAAACAGAATTTGCGACATTGATTTCT
GTCGACTTAAATGATTATTTAAAAGATGTCCAACTTGATAAAAAAACAATCAAAATTCCACATTGGTTGAACGTGCGTGC
GACGAATGAAGGAATTAATTTTTCTAAAACGATGACTGAAGCATTAGCTCAAAAATTAAATGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A022N4U5