104728

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3083129..3083699 Replicon chromosome
Accession NZ_CP029065
Organism Mycobacterium tuberculosis strain TBMENG-03

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag DDF83_RS15290 Protein ID WP_003414166.1
Coordinates 3083129..3083485 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag DDF83_RS15295 Protein ID WP_003901465.1
Coordinates 3083469..3083699 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DDF83_RS15270 3078581..3080269 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
DDF83_RS15275 3080273..3080599 - 327 WP_003414157.1 hypothetical protein -
DDF83_RS15280 3080772..3081359 + 588 WP_003914429.1 DUF3558 family protein -
DDF83_RS15285 3081378..3083027 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
DDF83_RS15290 3083129..3083485 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
DDF83_RS15295 3083469..3083699 - 231 WP_003901465.1 antitoxin MazE Antitoxin
DDF83_RS15300 3083742..3084785 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
DDF83_RS15305 3084976..3085251 + 276 WP_003911993.1 DUF1778 domain-containing protein -
DDF83_RS21460 3085427..3085681 - 255 WP_003917684.1 hypothetical protein -
DDF83_RS15315 3085829..3086233 + 405 WP_003414181.1 hypothetical protein -
DDF83_RS15320 3086230..3086421 + 192 WP_003414184.1 hypothetical protein -
DDF83_RS15325 3086485..3087774 + 1290 Protein_2893 transposase family protein -
DDF83_RS15330 3088008..3088265 + 258 WP_003899489.1 hypothetical protein -
DDF83_RS15335 3088370..3088681 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T104728 WP_003414166.1 NZ_CP029065:c3083485-3083129 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T104728 NZ_CP029065:c3083485-3083129 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT104728 WP_003901465.1 NZ_CP029065:c3083699-3083469 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT104728 NZ_CP029065:c3083699-3083469 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FBW3

References