Detailed information of TA system TA104727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3043546..3044233
• Replicon: chromosome
• Accession: NZ_CP029065
• Organism: Mycobacterium tuberculosis strain TBMENG-03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: DDF83_RS15075
• Protein ID: WP_003414064.1
• Coordinates: 3043838..3044233 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: DDF83_RS15070
• Protein ID: WP_003414061.1
• Coordinates: 3043546..3043812 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDF83_RS15045	3039185..3040087	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
DDF83_RS15050	3040156..3040908	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
DDF83_RS15055	3041152..3041427	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
DDF83_RS15060	3041424..3043046	-	1623	WP_031738739.1	type I restriction-modification system subunit M	-
DDF83_RS15065	3043133..3043549	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
DDF83_RS15070	3043546..3043812	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DDF83_RS15075	3043838..3044233	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
DDF83_RS15080	3044230..3044499	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
DDF83_RS15085	3044509..3045603	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
DDF83_RS15090	3045600..3046019	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
DDF83_RS15095	3046093..3046572	-	480	WP_003414073.1	dihydrofolate reductase	-
DDF83_RS15100	3046643..3047443	-	801	WP_003911953.1	thymidylate synthase	-
DDF83_RS15105	3047599..3048336	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T104727 WP_003414064.1 NZ_CP029065:c3044233-3043838 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T104727 NZ_CP029065:c3044233-3043838 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT104727 WP_003414061.1 NZ_CP029065:c3043812-3043546 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT104727 NZ_CP029065:c3043812-3043546 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64