Detailed information of TA system TA104716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2305447..2306083
• Replicon: chromosome
• Accession: NZ_CP029065
• Organism: Mycobacterium tuberculosis strain TBMENG-03

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DDF83_RS11255
• Protein ID: WP_003904769.1
• Coordinates: 2305673..2306083 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P9WJ84
• Locus tag: DDF83_RS11250
• Protein ID: WP_003410651.1
• Coordinates: 2305447..2305680 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDF83_RS11240	2301297..2301701	-	405	WP_003410645.1	PPOX class F420-dependent oxidoreductase	-
DDF83_RS11245	2301785..2305369	-	3585	WP_003410648.1	cobaltochelatase subunit CobN	-
DDF83_RS11250	2305447..2305680	+	234	WP_003410651.1	type II toxin-antitoxin system antitoxin MazE7	Antitoxin
DDF83_RS11255	2305673..2306083	+	411	WP_003904769.1	type II toxin-antitoxin system toxin endoribonuclease MazF7	Toxin
DDF83_RS11260	2306067..2307158	+	1092	WP_003900454.1	precorrin-3B synthase	-
DDF83_RS11265	2307168..2307794	+	627	WP_003410658.1	precorrin-8X methylmutase	-
DDF83_RS11270	2307791..2309317	+	1527	WP_003410659.1	precorrin-2 C(20)-methyltransferase	-
DDF83_RS11275	2309263..2310486	-	1224	WP_003901321.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14176.35 Da
• Isoelectric Point: 9.4835

>T104716 WP_003904769.1 NZ_CP029065:2305673-2306083 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMPAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Nucleotide

• Length: 411 bp

>T104716 NZ_CP029065:2305673-2306083 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCCCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8450.25 Da
• Isoelectric Point: 4.7954

>AT104716 WP_003410651.1 NZ_CP029065:2305447-2305680 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Nucleotide

• Length: 234 bp

>AT104716 NZ_CP029065:2305447-2305680 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV50