Detailed information of TA system TA104699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1226383..1226951
• Replicon: chromosome
• Accession: NZ_CP029065
• Organism: Mycobacterium tuberculosis strain TBMENG-03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: DDF83_RS06085
• Protein ID: WP_003405865.1
• Coordinates: 1226577..1226951 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: DDF83_RS06080
• Protein ID: WP_003405863.1
• Coordinates: 1226383..1226580 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDF83_RS06060	1222424..1223062	-	639	WP_003898730.1	lipid droplet-associated protein	-
DDF83_RS06065	1223152..1224159	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
DDF83_RS06070	1224176..1225159	-	984	WP_003903326.1	hypothetical protein	-
DDF83_RS06075	1225222..1226295	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
DDF83_RS06080	1226383..1226580	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DDF83_RS06085	1226577..1226951	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
DDF83_RS06090	1227154..1227852	+	699	WP_003898733.1	hypothetical protein	-
DDF83_RS06095	1227970..1228155	+	186	WP_003901093.1	hypothetical protein	-
DDF83_RS06100	1228082..1228357	-	276	WP_003405867.1	hypothetical protein	-
DDF83_RS06105	1228600..1228923	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
DDF83_RS06110	1228938..1229648	-	711	WP_003911399.1	hypothetical protein	-
DDF83_RS21635	1229670..1229879	+	210	WP_003911400.1	hypothetical protein	-
DDF83_RS06115	1229831..1230538	-	708	Protein_1155	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T104699 WP_003405865.1 NZ_CP029065:1226577-1226951 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T104699 NZ_CP029065:1226577-1226951 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT104699 WP_003405863.1 NZ_CP029065:1226383-1226580 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT104699 NZ_CP029065:1226383-1226580 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07