Detailed information of TA system TA104686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 699799..700442
• Replicon: chromosome
• Accession: NZ_CP029065
• Organism: Mycobacterium tuberculosis strain TBMENG-03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: DDF83_RS03250
• Protein ID: WP_003403187.1
• Coordinates: 700041..700442 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: DDF83_RS03245
• Protein ID: WP_003403184.1
• Coordinates: 699799..700044 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDF83_RS03210	695079..695609	-	531	WP_003917320.1	two component system sensor kinase HK2	-
DDF83_RS03215	695593..696288	-	696	WP_003918099.1	two-component system response regulator TcrA	-
DDF83_RS03220	696411..696722	+	312	WP_003403164.1	hypothetical protein	-
DDF83_RS03225	696794..697744	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
DDF83_RS03230	697985..698569	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
DDF83_RS03235	698571..699284	+	714	Protein_625	IS607 family element transposase accessory protein TnpB	-
DDF83_RS03240	699284..699754	+	471	WP_003898523.1	hypothetical protein	-
DDF83_RS03245	699799..700044	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DDF83_RS03250	700041..700442	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
DDF83_RS03265	700830..700997	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
DDF83_RS03270	701030..701227	-	198	WP_003403191.1	hypothetical protein	-
DDF83_RS03275	701550..702464	-	915	WP_003908555.1	hypothetical protein	-
DDF83_RS03280	702516..702737	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
DDF83_RS03285	702879..703484	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T104686 WP_003403187.1 NZ_CP029065:700041-700442 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T104686 NZ_CP029065:700041-700442 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT104686 WP_003403184.1 NZ_CP029065:699799-700044 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT104686 NZ_CP029065:699799-700044 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91