Detailed information of TA system TA104676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 11723..12245
• Replicon: plasmid pFORC_081_4
• Accession: NZ_CP029061
• Organism: Escherichia coli strain FORC_081

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A9LK42
• Locus tag: FORC81_RS25570
• Protein ID: WP_000220562.1
• Coordinates: 11964..12245 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: FORC81_RS25565
• Protein ID: WP_000121743.1
• Coordinates: 11723..11974 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC81_RS25525	6802..7032	+	231	WP_000051064.1	partitioning protein	-
FORC81_RS25530	7049..7360	+	312	WP_000360321.1	hypothetical protein	-
FORC81_RS25535	7398..8018	+	621	WP_012477163.1	hypothetical protein	-
FORC81_RS25540	8043..8678	+	636	WP_012477164.1	hypothetical protein	-
FORC81_RS25545	8709..9131	-	423	WP_001467132.1	DNA distortion polypeptide 3	-
FORC81_RS25550	9168..9614	-	447	WP_001074382.1	hypothetical protein	-
FORC81_RS25555	9654..10490	-	837	WP_001802123.1	replication initiation protein	-
FORC81_RS25565	11723..11974	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
FORC81_RS25570	11964..12245	+	282	WP_000220562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC81_RS25575	12391..12714	+	324	WP_001181905.1	hypothetical protein	-
FORC81_RS25580	12762..13007	+	246	WP_000356542.1	hypothetical protein	-
FORC81_RS25585	12997..13212	+	216	WP_001180117.1	hypothetical protein	-
FORC81_RS25590	13305..13634	+	330	WP_000866654.1	hypothetical protein	-
FORC81_RS25595	13676..13855	+	180	WP_000439079.1	hypothetical protein	-
FORC81_RS25885	13925..14074	+	150	WP_000003880.1	hypothetical protein	-
FORC81_RS25600	14087..14359	+	273	WP_000160399.1	hypothetical protein	-
FORC81_RS26005	14616..14690	+	75	Protein_25	DNA distortion polypeptide 3	-
FORC81_RS25605	14685..15230	+	546	WP_020801480.1	hypothetical protein	-
FORC81_RS25610	15233..16399	+	1167	WP_012477147.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32945	32945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11021.83 Da
• Isoelectric Point: 10.5938

>T104676 WP_000220562.1 NZ_CP029061:11964-12245 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVISVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T104676 NZ_CP029061:11964-12245 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTTGTTATTTCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT104676 WP_000121743.1 NZ_CP029061:11723-11974 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT104676 NZ_CP029061:11723-11974 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A9LK42

Antitoxin

• AlphaFold DB: A0A3S5I301