Detailed information of TA system TA104675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 22248..22840
• Replicon: plasmid pFORC_081_2
• Accession: NZ_CP029059
• Organism: Escherichia coli strain FORC_081

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: FORC81_RS25055
• Protein ID: WP_000513532.1
• Coordinates: 22248..22496 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: FORC81_RS25060
• Protein ID: WP_000681609.1
• Coordinates: 22493..22840 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC81_RS25015	17252..17917	-	666	WP_000532148.1	P-loop NTPase	-
FORC81_RS25025	18506..18835	+	330	WP_000855119.1	hypothetical protein	-
FORC81_RS25030	19239..19754	+	516	WP_001025393.1	J domain-containing protein	-
FORC81_RS25035	19858..20400	+	543	WP_001083904.1	hypothetical protein	-
FORC81_RS25040	20397..20651	+	255	WP_001230709.1	hypothetical protein	-
FORC81_RS25045	20648..20890	+	243	WP_000151456.1	hypothetical protein	-
FORC81_RS25050	21461..21754	+	294	WP_000673428.1	hypothetical protein	-
FORC81_RS25055	22248..22496	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
FORC81_RS25060	22493..22840	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FORC81_RS25065	22928..23110	-	183	WP_001428470.1	hypothetical protein	-
FORC81_RS25070	23612..23911	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
FORC81_RS25075	24171..24497	-	327	WP_000206697.1	hypothetical protein	-
FORC81_RS25080	24499..24939	-	441	WP_023223352.1	hypothetical protein	-
FORC81_RS25085	24959..25666	-	708	WP_000108794.1	hypothetical protein	-
FORC81_RS25090	25668..26420	-	753	WP_000797966.1	conjugal transfer protein TraL	-
FORC81_RS25095	26438..26839	-	402	WP_021575837.1	hypothetical protein	-
FORC81_RS25100	27208..27549	+	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61917	61917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T104675 WP_000513532.1 NZ_CP029059:22248-22496 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T104675 NZ_CP029059:22248-22496 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT104675 WP_000681609.1 NZ_CP029059:22493-22840 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT104675 NZ_CP029059:22493-22840 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49