104674

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 20397..20890 Replicon plasmid pFORC_081_2
Accession NZ_CP029059
Organism Escherichia coli strain FORC_081

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag FORC81_RS25040 Protein ID WP_001230709.1
Coordinates 20397..20651 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag FORC81_RS25045 Protein ID WP_000151456.1
Coordinates 20648..20890 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC81_RS25000 15969..16130 + 162 WP_106479314.1 type I toxin-antitoxin system Hok family toxin -
FORC81_RS25005 16280..16876 + 597 WP_000125165.1 hypothetical protein -
FORC81_RS25995 16894..17178 - 285 WP_001162371.1 hypothetical protein -
FORC81_RS25015 17252..17917 - 666 WP_000532148.1 P-loop NTPase -
FORC81_RS25025 18506..18835 + 330 WP_000855119.1 hypothetical protein -
FORC81_RS25030 19239..19754 + 516 WP_001025393.1 J domain-containing protein -
FORC81_RS25035 19858..20400 + 543 WP_001083904.1 hypothetical protein -
FORC81_RS25040 20397..20651 + 255 WP_001230709.1 hypothetical protein Toxin
FORC81_RS25045 20648..20890 + 243 WP_000151456.1 hypothetical protein Antitoxin
FORC81_RS25050 21461..21754 + 294 WP_000673428.1 hypothetical protein -
FORC81_RS25055 22248..22496 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
FORC81_RS25060 22493..22840 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
FORC81_RS25065 22928..23110 - 183 WP_001428470.1 hypothetical protein -
FORC81_RS25070 23612..23911 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
FORC81_RS25075 24171..24497 - 327 WP_000206697.1 hypothetical protein -
FORC81_RS25080 24499..24939 - 441 WP_023223352.1 hypothetical protein -
FORC81_RS25085 24959..25666 - 708 WP_000108794.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..61917 61917


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Antitoxin MqsA/HigA-2

(1-131)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T104674 WP_001230709.1 NZ_CP029059:20397-20651 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T104674 NZ_CP029059:20397-20651 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT104674 WP_000151456.1 NZ_CP029059:20648-20890 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT104674 NZ_CP029059:20648-20890 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References