Detailed information of TA system TA104673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 4120..4642
• Replicon: plasmid pFORC_081_2
• Accession: NZ_CP029059
• Organism: Escherichia coli strain FORC_081

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: FORC81_RS24905
• Protein ID: WP_000638823.1
• Coordinates: 4120..4401 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: FORC81_RS24910
• Protein ID: WP_000121741.1
• Coordinates: 4391..4642 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC81_RS24875	173..757	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
FORC81_RS24880	778..1176	-	399	WP_001153665.1	hypothetical protein	-
FORC81_RS24885	1316..2013	+	698	Protein_3	IS1 family transposase	-
FORC81_RS24890	2072..2509	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
FORC81_RS24895	2515..3750	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
FORC81_RS24900	3753..4052	-	300	WP_000835764.1	IncN plasmid KikA protein	-
FORC81_RS24905	4120..4401	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC81_RS24910	4391..4642	-	252	WP_000121741.1	hypothetical protein	Antitoxin
FORC81_RS24915	4742..5377	-	636	WP_015059536.1	hypothetical protein	-
FORC81_RS24920	5425..6257	+	833	Protein_10	DNA primase	-
FORC81_RS24925	6471..6695	-	225	WP_000713561.1	EexN family lipoprotein	-
FORC81_RS24930	6704..7348	-	645	WP_001310442.1	conjugal transfer protein TrbJ	-
FORC81_RS24935	7354..8349	-	996	WP_001028541.1	type IV secretion system protein	-
FORC81_RS24940	8353..8610	-	258	WP_134162616.1	hypothetical protein	-
FORC81_RS24955	9179..9391	-	213	WP_039022940.1	hypothetical protein	-
FORC81_RS25850	9402..9572	-	171	WP_000550720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61917	61917
	-	flank	IS/Tn	-	-	1316..1591	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T104673 WP_000638823.1 NZ_CP029059:c4401-4120 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T104673 NZ_CP029059:c4401-4120 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT104673 WP_000121741.1 NZ_CP029059:c4642-4391 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT104673 NZ_CP029059:c4642-4391 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1