Detailed information of TA system TA104634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2879254..2879891
• Replicon: chromosome
• Accession: NZ_CP029034
• Organism: Bacillus velezensis strain LDO2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: DDE72_RS14210
• Protein ID: WP_003156187.1
• Coordinates: 2879254..2879604 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: DDE72_RS14215
• Protein ID: WP_003156188.1
• Coordinates: 2879610..2879891 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDE72_RS14170	2874294..2874896	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
DDE72_RS14175	2874896..2875684	-	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
DDE72_RS14180	2875650..2876132	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
DDE72_RS14185	2876129..2876458	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
DDE72_RS14190	2876522..2877529	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
DDE72_RS14195	2877541..2877942	-	402	WP_003156178.1	anti-sigma regulatory factor	-
DDE72_RS14200	2877945..2878310	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
DDE72_RS14205	2878315..2879136	-	822	WP_003156182.1	STAS domain-containing protein	-
DDE72_RS14210	2879254..2879604	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
DDE72_RS14215	2879610..2879891	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
DDE72_RS14220	2880011..2881180	-	1170	WP_032873001.1	alanine racemase	-
DDE72_RS14225	2881297..2882304	-	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
DDE72_RS14230	2882469..2882834	-	366	WP_007609580.1	holo-ACP synthase	-
DDE72_RS14235	2882927..2883526	+	600	WP_032872997.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T104634 WP_003156187.1 NZ_CP029034:c2879604-2879254 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T104634 NZ_CP029034:c2879604-2879254 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT104634 WP_003156188.1 NZ_CP029034:c2879891-2879610 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT104634 NZ_CP029034:c2879891-2879610 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5