104587

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 1797..2382 Replicon plasmid pAbW39-2
Accession NZ_CP029007
Organism Acinetobacter pittii strain AbW39

Toxin (Protein)


Gene name brnT Uniprot ID A0A158LPL9
Locus tag DDE02_RS20720 Protein ID WP_002124950.1
Coordinates 2095..2382 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A158LPT9
Locus tag DDE02_RS20715 Protein ID WP_004836815.1
Coordinates 1797..2108 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DDE02_RS20700 (DDE02_20975) 117..644 + 528 WP_002124964.1 plasmid pRiA4b ORF-3 family protein -
DDE02_RS20705 (DDE02_20980) 854..1210 - 357 WP_020992414.1 hypothetical protein -
DDE02_RS20710 (DDE02_20985) 1324..1710 + 387 WP_006581720.1 hypothetical protein -
DDE02_RS20715 (DDE02_20990) 1797..2108 - 312 WP_004836815.1 BrnA antitoxin family protein Antitoxin
DDE02_RS20720 (DDE02_20995) 2095..2382 - 288 WP_002124950.1 BrnT family toxin Toxin
DDE02_RS20725 (DDE02_21000) 2612..2929 - 318 WP_020992415.1 hypothetical protein -
DDE02_RS20730 (DDE02_21005) 3287..4171 - 885 WP_006581719.1 MobA/MobL family protein -
DDE02_RS20735 (DDE02_21010) 4627..5340 + 714 WP_006581718.1 hypothetical protein -
DDE02_RS20740 (DDE02_21015) 6040..6969 + 930 WP_005065049.1 replication initiation protein RepM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..149504 149504


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11173.58 Da        Isoelectric Point: 4.9093

>T104587 WP_002124950.1 NZ_CP029007:c2382-2095 [Acinetobacter pittii]
MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNGEERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIIS
ARQVTKAERNQYEHG

Download         Length: 288 bp

>T104587 NZ_CP029007:c2382-2095 [Acinetobacter pittii]
ATGGAACTGTATTTCGAATGGGATGAGGCTAAAAACCAAAAGAATCAGAAAAAACATGATGTATCTTTTGAAACGGCAAG
CCTCGTTTTTGAAGATCCTTTACGGATCTCAATCCAGGATCGACATACCAATGGTGAAGAACGTTGGCAAACGATCGGTA
TGGTTAAAGGTGTACTGATGTTATTGGTGGCTCATACAATCATTGACGATGATGACTGTGAGATCATACGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAACGCAATCAATATGAGCATGGTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 12056.73 Da        Isoelectric Point: 8.5036

>AT104587 WP_004836815.1 NZ_CP029007:c2108-1797 [Acinetobacter pittii]
MSMVRYTRKELNENFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEITDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQ
GKGYQTRMNKILREAMLEDLKNH

Download         Length: 312 bp

>AT104587 NZ_CP029007:c2108-1797 [Acinetobacter pittii]
ATGAGCATGGTTAGATATACACGCAAAGAACTGAATGAAAATTTCAGTGACAAGCAAGATGCTGAAATTAAACGCTTGCT
TGCGAAAGGAACTGTACCAGATGAACAATTAGATCTATCTGACATTCCTGAGATTACCGACTGGAGTCATGCTGTTCGCC
ATGGGCAATTTTATCGTCCAGTGAAGCAACAAACATCTGTTCGTCTAGATGCAGATGTACTTGCATGGTTAAAGGCTCAA
GGCAAAGGCTATCAGACACGCATGAATAAAATTCTGCGTGAAGCAATGCTAGAAGATCTTAAGAATCATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A158LPL9

Antitoxin

Source ID Structure
AlphaFold DB A0A158LPT9

References