Detailed information of TA system TA104583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2093520..2094235
• Replicon: chromosome
• Accession: NZ_CP029003
• Organism: Actinobacillus pleuropneumoniae serovar

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: APPSER1_RS09980
• Protein ID: WP_005619039.1
• Coordinates: 2093520..2093948 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: APPSER1_RS09985
• Protein ID: WP_005599447.1
• Coordinates: 2093948..2094235 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APPSER1_RS09965	2089105..2090811	-	1707	WP_005599433.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
APPSER1_RS09970	2090907..2091188	-	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
APPSER1_RS09975	2091315..2093396	-	2082	WP_005599444.1	ATP-dependent DNA helicase RecG	-
APPSER1_RS09980	2093520..2093948	-	429	WP_005619039.1	type II toxin-antitoxin system VapC family toxin	Toxin
APPSER1_RS09985	2093948..2094235	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
APPSER1_RS09990	2094441..2096156	+	1716	WP_005599448.1	proline--tRNA ligase	-
APPSER1_RS09995	2096222..2096410	-	189	WP_005602684.1	hypothetical protein	-
APPSER1_RS10000	2096413..2096994	-	582	WP_005599450.1	sigma-70 family RNA polymerase sigma factor	-
APPSER1_RS10005	2097083..2098039	+	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
APPSER1_RS10010	2098039..2098632	+	594	WP_005599453.1	sulfoxide reductase heme-binding subunit YedZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16508.97 Da
• Isoelectric Point: 8.9724

>T104583 WP_005619039.1 NZ_CP029003:c2093948-2093520 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
MFQYLFDTNIISELYKLGSNRMDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQA
KSFSINNEIALLASEYHIPNKMDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 429 bp

>T104583 NZ_CP029003:c2093948-2093520 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
ATGTTTCAATATCTTTTTGATACCAACATCATTTCCGAGCTTTATAAGCTAGGAAGCAATAGAATGGATACCAATGTACG
ACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAATAAAAACGGGGATTTTAC
TCAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTATTAAGCGTTTATCAAGCT
AAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAAATGGATTTAAACGATGC
TTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTTCAACCGTTGCGATATAC
GTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT104583 WP_005599447.1 NZ_CP029003:c2094235-2093948 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT104583 NZ_CP029003:c2094235-2093948 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5