Detailed information of TA system TA104581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1796961..1797692
• Replicon: chromosome
• Accession: NZ_CP029003
• Organism: Actinobacillus pleuropneumoniae serovar

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: APPSER1_RS08405
• Protein ID: WP_005598840.1
• Coordinates: 1796961..1797428 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: APPSER1_RS08410
• Protein ID: WP_005619269.1
• Coordinates: 1797432..1797692 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APPSER1_RS08380	1792320..1792691	-	372	WP_005598830.1	CrcB family protein	-
APPSER1_RS08385	1792694..1793758	-	1065	WP_043991897.1	MFS transporter	-
APPSER1_RS08390	1793853..1794962	+	1110	WP_005598834.1	anhydro-N-acetylmuramic acid kinase	-
APPSER1_RS08395	1795016..1795930	+	915	WP_005598836.1	N-acetylmuramic acid 6-phosphate etherase	-
APPSER1_RS08400	1795997..1796878	-	882	WP_005598838.1	50S ribosomal protein L11 methyltransferase	-
APPSER1_RS08405	1796961..1797428	-	468	WP_005598840.1	GNAT family N-acetyltransferase	Toxin
APPSER1_RS08410	1797432..1797692	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
APPSER1_RS08415	1797813..1799273	-	1461	WP_005598844.1	metalloprotease TldD	-
APPSER1_RS08420	1799403..1800662	+	1260	WP_005598846.1	tRNA lysidine(34) synthetase TilS	-
APPSER1_RS08425	1800689..1800979	-	291	WP_005598848.1	DUF5389 family protein	-
APPSER1_RS08430	1800981..1801874	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17206.22 Da
• Isoelectric Point: 8.9312

>T104581 WP_005598840.1 NZ_CP029003:c1797428-1796961 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGIKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T104581 NZ_CP029003:c1797428-1796961 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAAAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGTGCCTTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGATAAAAGCATTGCTTGTTCATGCGTTAAACCCGCAAGCAAAAC
AATTTTATTTAAAGTATGGTTTTTCATGTTCACCCATTGATGAGATGGTATTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT104581 WP_005619269.1 NZ_CP029003:c1797692-1797432 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT104581 NZ_CP029003:c1797692-1797432 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A3N2I7