Detailed information of TA system TA104580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 1588628..1589273
• Replicon: chromosome
• Accession: NZ_CP029003
• Organism: Actinobacillus pleuropneumoniae serovar

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: B0BQU7
• Locus tag: APPSER1_RS07405
• Protein ID: WP_005619674.1
• Coordinates: 1588902..1589273 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: APPSER1_RS07400
• Protein ID: WP_005598477.1
• Coordinates: 1588628..1588921 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APPSER1_RS07370	1583969..1584652	-	684	WP_005598468.1	arginine ABC transporter permease ArtM	-
APPSER1_RS07375	1584652..1585323	-	672	WP_005598470.1	arginine ABC transporter permease ArtQ	-
APPSER1_RS07380	1585329..1586048	-	720	WP_043991873.1	transporter substrate-binding domain-containing protein	-
APPSER1_RS07385	1586068..1586802	-	735	WP_005598473.1	arginine ABC transporter ATP-binding protein ArtP	-
APPSER1_RS07390	1587027..1587524	-	498	WP_005598474.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
APPSER1_RS07395	1587597..1588550	+	954	WP_043991874.1	calcium/sodium antiporter	-
APPSER1_RS07400	1588628..1588921	-	294	WP_005598477.1	helix-turn-helix domain-containing protein	Antitoxin
APPSER1_RS07405	1588902..1589273	-	372	WP_005619674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
APPSER1_RS07410	1589467..1591218	+	1752	WP_005598482.1	protein-disulfide reductase DsbD	-
APPSER1_RS07415	1591276..1591845	+	570	WP_005598484.1	elongation factor P hydroxylase	-
APPSER1_RS07420	1591889..1592743	-	855	WP_005598486.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 15066.57 Da
• Isoelectric Point: 10.4054

>T104580 WP_005619674.1 NZ_CP029003:c1589273-1588902 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
MYEIVFYRDKRGREPVKEFLLRLLKERQEGSRERLHKISHHLSILHLHGTRAGENYIKHLEDRIWQLRPVGDCLLFASII
RGKFVLLHYFAKQNYRIPKREIERAKVRLADLQERIKDEPYWF

Nucleotide

• Length: 372 bp

>T104580 NZ_CP029003:c1589273-1588902 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
ATGTATGAAATAGTCTTTTATCGAGATAAGCGGGGGAGAGAACCCGTAAAAGAATTTCTGTTAAGGCTACTAAAAGAACG
GCAGGAAGGCAGTCGAGAACGATTACACAAAATCAGCCATCATTTGAGTATTTTACATTTGCACGGTACTCGAGCCGGTG
AGAATTATATTAAGCATTTGGAAGATCGTATTTGGCAGTTACGTCCGGTGGGCGATTGCCTGTTATTTGCAAGTATTATT
CGGGGAAAATTTGTGTTGTTGCATTATTTTGCCAAACAGAATTATCGAATTCCGAAACGAGAAATAGAACGTGCAAAAGT
TCGGTTGGCGGATTTACAGGAGAGAATAAAAGATGAGCCCTATTGGTTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10935.81 Da
• Isoelectric Point: 10.3120

>AT104580 WP_005598477.1 NZ_CP029003:c1588921-1588628 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
MSPIGSSWTSFEQEIFDESEIAETQFRIRLILEIIETRQQLGISQRKLEKLSGVKQSMIARVEKGSVNPSLATVLKLLVP
LGKTLQIVPLDKNKTVR

Nucleotide

• Length: 294 bp

>AT104580 NZ_CP029003:c1588921-1588628 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
ATGAGCCCTATTGGTTCTAGTTGGACATCGTTTGAACAAGAAATTTTTGACGAGTCGGAAATAGCGGAAACTCAGTTTCG
CATACGGCTTATTTTAGAAATCATTGAAACCCGTCAGCAGCTCGGTATCTCTCAACGAAAATTGGAGAAATTAAGCGGCG
TAAAACAATCTATGATTGCTCGGGTAGAAAAAGGCTCGGTTAATCCGTCGTTGGCGACGGTATTAAAGTTGCTTGTGCCG
TTGGGGAAAACTTTACAAATCGTACCGTTAGATAAAAACAAAACGGTACGTTAA

Structures

Toxin

• AlphaFold DB: A3N206