Detailed information of TA system TA104579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1502533..1503041
• Replicon: chromosome
• Accession: NZ_CP029003
• Organism: Actinobacillus pleuropneumoniae serovar

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: APPSER1_RS06950
• Protein ID: WP_005605064.1
• Coordinates: 1502781..1503041 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: APPSER1_RS06945
• Protein ID: WP_005598318.1
• Coordinates: 1502533..1502784 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APPSER1_RS06905	1498070..1498618	-	549	WP_005598298.1	type IV pilus biogenesis/stability protein PilW	-
APPSER1_RS06910	1498672..1499853	-	1182	WP_005598300.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
APPSER1_RS06935	1500757..1502196	+	1440	WP_005598316.1	glutamate--tRNA ligase	-
APPSER1_RS06945	1502533..1502784	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
APPSER1_RS06950	1502781..1503041	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
APPSER1_RS06955	1503200..1503367	-	168	WP_005598320.1	Trm112 family protein	-
APPSER1_RS06960	1503369..1504349	-	981	WP_005598322.1	tetraacyldisaccharide 4'-kinase	-
APPSER1_RS06965	1504443..1505702	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
APPSER1_RS06970	1505702..1506292	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
APPSER1_RS06975	1506414..1506806	+	393	WP_005620423.1	SufE family protein	-
APPSER1_RS06990	1507165..1507938	-	774	WP_005598329.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T104579 WP_005605064.1 NZ_CP029003:1502781-1503041 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T104579 NZ_CP029003:1502781-1503041 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT104579 WP_005598318.1 NZ_CP029003:1502533-1502784 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT104579 NZ_CP029003:1502533-1502784 [Actinobacillus pleuropneumoniae serovar 1 str. 4074]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8