104559

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 587195..587820 Replicon chromosome
Accession NZ_CP028994
Organism Klebsiella pneumoniae strain AR_0079

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag AM458_RS03015 Protein ID WP_002882817.1
Coordinates 587437..587820 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AM458_RS03010 Protein ID WP_004150355.1
Coordinates 587195..587437 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM458_RS02985 583186..583785 + 600 WP_004151865.1 glucose-1-phosphatase -
AM458_RS02990 583779..584639 + 861 WP_002882807.1 virulence factor BrkB family protein -
AM458_RS02995 584636..585073 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AM458_RS03000 585118..586059 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AM458_RS03005 586073..586990 - 918 WP_108714542.1 alpha/beta hydrolase -
AM458_RS03010 587195..587437 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AM458_RS03015 587437..587820 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
AM458_RS03020 587994..588923 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AM458_RS03025 588920..589555 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AM458_RS03030 589552..590454 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T104559 WP_002882817.1 NZ_CP028994:587437-587820 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T104559 NZ_CP028994:587437-587820 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT104559 WP_004150355.1 NZ_CP028994:587195-587437 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT104559 NZ_CP028994:587195-587437 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References