Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17218..17861 Replicon plasmid unnamed1
Accession NZ_CP028991
Organism Klebsiella pneumoniae strain AR_0142

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag AM389_RS26845 Protein ID WP_000754566.1
Coordinates 17445..17861 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag AM389_RS26840 Protein ID WP_001261276.1
Coordinates 17218..17448 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM389_RS26795 12404..12877 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
AM389_RS26800 12969..13199 + 231 WP_011977773.1 hypothetical protein -
AM389_RS26805 14091..14873 - 783 WP_004152340.1 site-specific integrase -
AM389_RS26810 14873..15205 - 333 WP_004152339.1 hypothetical protein -
AM389_RS26815 15212..15610 - 399 WP_004171440.1 hypothetical protein -
AM389_RS26820 15636..15965 - 330 WP_004152337.1 hypothetical protein -
AM389_RS26825 15993..16301 - 309 WP_004152336.1 hypothetical protein -
AM389_RS26840 17218..17448 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM389_RS26845 17445..17861 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
AM389_RS26850 17902..18779 - 878 Protein_18 restriction endonuclease -
AM389_RS26855 19438..20715 + 1278 WP_016338369.1 HlyD family secretion protein -
AM389_RS26860 20705..22813 + 2109 WP_016338368.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul2 / aac(6')-Ib-cr / dfrA14 - 1..85698 85698
- flank IS/Tn - - 11016..12284 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T104556 WP_000754566.1 NZ_CP028991:17445-17861 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T104556 NZ_CP028991:17445-17861 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT104556 WP_001261276.1 NZ_CP028991:17218-17448 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT104556 NZ_CP028991:17218-17448 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References