Detailed information of TA system TA104537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 143748..144433
• Replicon: plasmid pUSP2
• Accession: NZ_CP028970
• Organism: Aminobacter sp. MSH1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DCS14_RS29195
• Protein ID: WP_108610498.1
• Coordinates: 144011..144433 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DCS14_RS29190
• Protein ID: WP_108610497.1
• Coordinates: 143748..144014 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCS14_RS29165	138946..140174	+	1229	WP_108610493.1	IS3 family transposase	-
DCS14_RS29170	140212..140304	-	93	Protein_129	Fic family protein	-
DCS14_RS29175	140526..141257	-	732	WP_108610494.1	SMC-Scp complex subunit ScpB	-
DCS14_RS29180	141263..142294	-	1032	WP_108610495.1	DUF1403 family protein	-
DCS14_RS29185	142420..143658	+	1239	WP_108610496.1	site-specific integrase	-
DCS14_RS29190	143748..144014	+	267	WP_108610497.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DCS14_RS29195	144011..144433	+	423	WP_108610498.1	type II toxin-antitoxin system VapC family toxin	Toxin
DCS14_RS29200	144664..144873	+	210	WP_108610499.1	hypothetical protein	-
DCS14_RS29205	144946..146262	-	1317	WP_108610500.1	replication initiation protein RepC	-
DCS14_RS29210	146485..147498	-	1014	WP_108610501.1	plasmid partitioning protein RepB	-
DCS14_RS29215	147495..148712	-	1218	WP_108610502.1	plasmid partitioning protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..365485	365485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-127)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-56)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15776.03 Da
• Isoelectric Point: 4.8158

>T104537 WP_108610498.1 NZ_CP028970:144011-144433 [Aminobacter sp. MSH1]
MRLLLDTNVLSEVTKQAPDPRVLEWLDQLDEDRSFISVVSIAEIRRGVALMDEGRKREALAEWLAQNLPQRFEQRVLHVD
EPIALAWGDLMAQAKRRGRGLSSMDGLIAATATAKQLTLATRNTKDFEGFAVELFDPWTG

Nucleotide

• Length: 423 bp

>T104537 NZ_CP028970:144011-144433 [Aminobacter sp. MSH1]
TTGAGGCTCTTGCTCGATACGAATGTCCTGTCTGAGGTTACGAAGCAGGCCCCCGACCCAAGGGTTCTGGAATGGCTGGA
CCAGCTCGATGAAGACCGTTCGTTCATCAGCGTGGTGTCGATTGCCGAAATCCGGCGCGGCGTCGCCCTCATGGACGAGG
GCCGGAAACGCGAGGCTTTGGCCGAATGGCTCGCGCAGAATCTGCCGCAGCGTTTCGAGCAAAGGGTTCTCCATGTTGAC
GAGCCTATTGCTCTGGCCTGGGGCGATCTGATGGCCCAGGCCAAGCGTCGCGGTCGTGGTCTGTCATCAATGGATGGCTT
AATTGCCGCGACAGCGACCGCGAAACAGCTCACCTTGGCCACGCGCAACACCAAGGATTTCGAAGGCTTCGCGGTCGAGC
TCTTCGACCCGTGGACTGGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9942.33 Da
• Isoelectric Point: 10.1733

>AT104537 WP_108610497.1 NZ_CP028970:143748-144014 [Aminobacter sp. MSH1]
MLSVPKDVAKWTVADAKARLSEVMERAQSVPQTITRNGKPSVVVVSAEEWQRKTAHRGTLAEFLMDSPLRSADLDLARQR
DEPRDQRL

Nucleotide

• Length: 267 bp

>AT104537 NZ_CP028970:143748-144014 [Aminobacter sp. MSH1]
ATGCTATCTGTACCCAAAGACGTTGCCAAATGGACCGTCGCCGACGCCAAAGCCCGGCTGTCGGAGGTTATGGAGCGCGC
GCAGTCCGTACCTCAAACGATCACCCGGAACGGCAAGCCAAGCGTCGTTGTCGTCTCGGCCGAGGAATGGCAGCGCAAGA
CGGCGCACAGGGGCACGCTGGCGGAGTTCCTTATGGACTCACCACTGCGTAGCGCCGATCTCGACCTCGCGCGTCAGCGC
GACGAACCGCGTGATCAGCGGCTTTGA