Detailed information of TA system TA104530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3971..4562
• Replicon: plasmid pBAM2
• Accession: NZ_CP028966
• Organism: Aminobacter sp. MSH1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: DCS14_RS00025
• Protein ID: WP_108605328.1
• Coordinates: 4266..4562 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DCS14_RS00020
• Protein ID: WP_108605327.1
• Coordinates: 3971..4264 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCS14_RS00010	1370..2374	+	1005	WP_108605325.1	plasmid partitioning protein RepB	-
DCS14_RS00015	2574..3890	+	1317	WP_108605326.1	replication initiation protein RepC	-
DCS14_RS00020	3971..4264	-	294	WP_108605327.1	putative addiction module antidote protein	Antitoxin
DCS14_RS00025	4266..4562	-	297	WP_108605328.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DCS14_RS00030	4625..5554	-	930	WP_108605371.1	recombinase family protein	-
DCS14_RS00035	5746..7428	+	1683	WP_108605329.1	DDE-type integrase/transposase/recombinase	-
DCS14_RS00040	7431..8330	+	900	WP_199914671.1	TniB family NTP-binding protein	-
DCS14_RS00045	8345..9487	+	1143	WP_159091756.1	TniQ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53893	53893

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (42-89)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11234.95 Da
• Isoelectric Point: 10.6190

>T104530 WP_108605328.1 NZ_CP028966:c4562-4266 [Aminobacter sp. MSH1]
MIELKQTETFRKWRARLKDQRVKALLASRLDRLAFGHAGDAEPVGQGISELRIHYGPGYRIYFRRHKDTIIILLCGGNKS
SQAKDIKTAKHLSAEWSD

Nucleotide

• Length: 297 bp

>T104530 NZ_CP028966:c4562-4266 [Aminobacter sp. MSH1]
ATGATCGAGCTTAAGCAGACCGAGACGTTTAGAAAATGGCGTGCGCGGCTCAAGGACCAGCGGGTAAAAGCGCTGCTCGC
TTCTCGTCTTGATCGACTGGCATTTGGTCACGCCGGAGATGCCGAGCCGGTCGGACAAGGGATCAGTGAGCTTCGAATTC
ACTACGGTCCCGGCTACCGGATCTATTTCCGACGACACAAAGATACAATCATTATCCTTTTGTGCGGTGGAAACAAAAGT
AGCCAGGCAAAGGACATCAAGACAGCAAAGCATCTTTCTGCAGAATGGAGCGACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10408.71 Da
• Isoelectric Point: 4.5837

>AT104530 WP_108605327.1 NZ_CP028966:c4264-3971 [Aminobacter sp. MSH1]
MAEKLTTYDPAEDLTDDDAIAVFMAQAFETGDAGYVAHALGVVARARGMSEIARETGLSREQLYRSFSESGNPTLRTTLA
VMRALGVDMTAKPHIAS

Nucleotide

• Length: 294 bp

>AT104530 NZ_CP028966:c4264-3971 [Aminobacter sp. MSH1]
ATGGCCGAGAAACTGACAACATACGATCCAGCGGAAGATTTGACCGATGATGACGCCATCGCTGTCTTTATGGCCCAGGC
CTTCGAGACAGGTGATGCTGGCTACGTCGCACACGCGCTCGGCGTTGTCGCACGTGCCAGAGGTATGAGCGAAATTGCCC
GGGAAACCGGCTTGTCGCGTGAACAGCTTTATCGCTCCTTCAGTGAAAGTGGGAATCCAACGCTCAGGACAACACTGGCC
GTCATGCGGGCCCTTGGCGTGGATATGACTGCAAAACCGCATATCGCTTCCTGA