Detailed information of TA system TA104514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 384055..384972
• Replicon: chromosome
• Accession: NZ_CP028961
• Organism: Bacillus velezensis strain SRCM102752

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: DBK22_RS02140
• Protein ID: WP_015239694.1
• Coordinates: 384226..384972 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: DBK22_RS02135
• Protein ID: WP_003154807.1
• Coordinates: 384055..384225 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBK22_RS02100	379267..380856	+	1590	WP_160241908.1	hypothetical protein	-
DBK22_RS02105	380869..381294	+	426	WP_160241909.1	hypothetical protein	-
DBK22_RS02110	381299..381496	+	198	WP_012117366.1	XkdX family protein	-
DBK22_RS02115	381553..382314	+	762	WP_160241910.1	phage portal protein	-
DBK22_RS02120	382366..382629	+	264	WP_003154815.1	hemolysin XhlA family protein	-
DBK22_RS02125	382643..382906	+	264	WP_003154813.1	phage holin	-
DBK22_RS02130	382920..383798	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
DBK22_RS02135	384055..384225	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
DBK22_RS02140	384226..384972	-	747	WP_015239694.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
DBK22_RS02145	385080..386078	-	999	WP_007407255.1	inorganic phosphate transporter	-
DBK22_RS02150	386091..386708	-	618	WP_003154804.1	DUF47 domain-containing protein	-
DBK22_RS02155	386995..388311	-	1317	WP_007610842.1	amino acid permease	-
DBK22_RS02160	388635..389585	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29135.61 Da
• Isoelectric Point: 4.7003

>T104514 WP_015239694.1 NZ_CP028961:c384972-384226 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T104514 NZ_CP028961:c384972-384226 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGAATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGAGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT104514 WP_003154807.1 NZ_CP028961:c384225-384055 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT104514 NZ_CP028961:c384225-384055 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14