Detailed information of TA system TA104512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5206509..5207095
• Replicon: chromosome
• Accession: NZ_CP028959
• Organism: Pseudomonas aeruginosa strain IMP66

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: DBX28_RS24860
• Protein ID: WP_003120987.1
• Coordinates: 5206796..5207095 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DBX28_RS24855
• Protein ID: WP_003448662.1
• Coordinates: 5206509..5206799 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBX28_RS24835	5201661..5201870	+	210	WP_003105733.1	cold-shock protein	-
DBX28_RS24840	5202091..5203980	+	1890	WP_003105732.1	hypothetical protein	-
DBX28_RS24845	5203977..5205953	+	1977	WP_017002277.1	DEAD/DEAH box helicase	-
DBX28_RS24850	5206094..5206438	+	345	WP_003448665.1	hypothetical protein	-
DBX28_RS24855	5206509..5206799	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
DBX28_RS24860	5206796..5207095	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBX28_RS24865	5207297..5208418	+	1122	WP_017002276.1	TcpQ domain-containing protein	-
DBX28_RS24870	5208418..5210127	+	1710	WP_020751093.1	PilN family type IVB pilus formation outer membrane protein	-
DBX28_RS24875	5210131..5211456	+	1326	WP_017002275.1	type 4b pilus protein PilO2	-
DBX28_RS24880	5211446..5211979	+	534	WP_017002274.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5179005..5291079	112074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T104512 WP_003120987.1 NZ_CP028959:c5207095-5206796 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T104512 NZ_CP028959:c5207095-5206796 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCTAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATTCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT104512 WP_003448662.1 NZ_CP028959:c5206799-5206509 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT104512 NZ_CP028959:c5206799-5206509 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAGTCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTAA

Structures

Toxin

• AlphaFold DB: G8CP73