Detailed information of TA system TA104511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5198538..5199178
• Replicon: chromosome
• Accession: NZ_CP028959
• Organism: Pseudomonas aeruginosa strain IMP66

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DBX28_RS24820
• Protein ID: WP_003105740.1
• Coordinates: 5198538..5198948 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: DBX28_RS24825
• Protein ID: WP_003158175.1
• Coordinates: 5198948..5199178 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBX28_RS24775	5194380..5194577	+	198	WP_003105756.1	hypothetical protein	-
DBX28_RS24780	5194733..5195461	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
DBX28_RS24785	5195467..5196015	+	549	WP_003105753.1	DUF3158 family protein	-
DBX28_RS24790	5196062..5196901	+	840	WP_025997473.1	Rha family transcriptional regulator	-
DBX28_RS24795	5196931..5197419	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
DBX28_RS24800	5197527..5197772	+	246	WP_023102333.1	CrpP family protein	-
DBX28_RS24805	5197838..5198056	-	219	WP_003105747.1	hypothetical protein	-
DBX28_RS24815	5198256..5198516	-	261	WP_003105742.1	hypothetical protein	-
DBX28_RS24820	5198538..5198948	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DBX28_RS24825	5198948..5199178	-	231	WP_003158175.1	antitoxin	Antitoxin
DBX28_RS24830	5199434..5201353	+	1920	WP_004352838.1	type I DNA topoisomerase	-
DBX28_RS24835	5201661..5201870	+	210	WP_003105733.1	cold-shock protein	-
DBX28_RS24840	5202091..5203980	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5179005..5291079	112074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T104511 WP_003105740.1 NZ_CP028959:c5198948-5198538 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T104511 NZ_CP028959:c5198948-5198538 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT104511 WP_003158175.1 NZ_CP028959:c5199178-5198948 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT104511 NZ_CP028959:c5199178-5198948 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2