Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 97311..97954 Replicon plasmid unnamed2
Accession NZ_CP028955
Organism Klebsiella pneumoniae strain AR_0141

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag AM388_RS28340 Protein ID WP_000754566.1
Coordinates 97311..97727 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag AM388_RS28345 Protein ID WP_001261276.1
Coordinates 97724..97954 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM388_RS28325 92359..94467 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
AM388_RS28330 94457..95734 - 1278 WP_016338369.1 HlyD family secretion protein -
AM388_RS28335 96393..97270 + 878 Protein_106 restriction endonuclease -
AM388_RS28340 97311..97727 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
AM388_RS28345 97724..97954 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM388_RS28360 98871..99179 + 309 WP_004152336.1 hypothetical protein -
AM388_RS28365 99207..99536 + 330 WP_004152337.1 hypothetical protein -
AM388_RS28370 99562..99960 + 399 WP_004171440.1 hypothetical protein -
AM388_RS28375 99967..100299 + 333 WP_004152339.1 hypothetical protein -
AM388_RS28380 100299..101081 + 783 WP_004152340.1 site-specific integrase -
AM388_RS28385 101973..102203 - 231 WP_011977773.1 hypothetical protein -
AM388_RS28390 102295..102768 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / dfrA14 / aac(6')-Ib-cr / sul2 - 1..134346 134346
- flank IS/Tn - - 102888..104156 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T104504 WP_000754566.1 NZ_CP028955:c97727-97311 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T104504 NZ_CP028955:c97727-97311 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT104504 WP_001261276.1 NZ_CP028955:c97954-97724 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT104504 NZ_CP028955:c97954-97724 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References