Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 141474..142210 Replicon plasmid unnamed1
Accession NZ_CP028954
Organism Klebsiella pneumoniae strain AR_0141

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM388_RS27330 Protein ID WP_003026803.1
Coordinates 141728..142210 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM388_RS27325 Protein ID WP_003026799.1
Coordinates 141474..141740 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM388_RS27280 137536..137898 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AM388_RS27285 137948..138298 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM388_RS27290 138656..138925 + 270 WP_004152102.1 hypothetical protein -
AM388_RS27295 138913..139488 + 576 WP_004152103.1 hypothetical protein -
AM388_RS27300 139519..140013 + 495 WP_004152104.1 DNA-binding protein -
AM388_RS27305 140057..140425 + 369 WP_004152105.1 hypothetical protein -
AM388_RS27310 140459..140662 + 204 WP_068814622.1 hemolysin expression modulator Hha -
AM388_RS27315 140711..140968 + 258 WP_004152107.1 hypothetical protein -
AM388_RS27320 141044..141298 + 255 WP_004152108.1 hypothetical protein -
AM388_RS27325 141474..141740 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM388_RS27330 141728..142210 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM388_RS27335 142418..143764 + 1347 WP_077253535.1 ISNCY family transposase -
AM388_RS27340 143813..144208 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
AM388_RS27345 144397..145794 + 1398 WP_009653207.1 ISNCY family transposase -
AM388_RS27350 145815..146006 + 192 Protein_153 hypothetical protein -
AM388_RS27355 146034..146312 - 279 WP_004197671.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / qacE / sul1 - 1..214704 214704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T104503 WP_003026803.1 NZ_CP028954:141728-142210 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T104503 NZ_CP028954:141728-142210 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT104503 WP_003026799.1 NZ_CP028954:141474-141740 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT104503 NZ_CP028954:141474-141740 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References