Detailed information of TA system TA104496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 3369697..3370507
• Replicon: chromosome
• Accession: NZ_CP028953
• Organism: Klebsiella pneumoniae strain AR_0141

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: AM388_RS17005
• Protein ID: WP_068815439.1
• Coordinates: 3369697..3370230 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: AM388_RS17010
• Protein ID: WP_002887278.1
• Coordinates: 3370241..3370507 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM388_RS17000	3368528..3369649	+	1122	WP_023280822.1	cupin domain-containing protein	-
AM388_RS17005	3369697..3370230	-	534	WP_068815439.1	GNAT family N-acetyltransferase	Toxin
AM388_RS17010	3370241..3370507	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
AM388_RS17015	3370610..3372043	-	1434	WP_068815441.1	Cu(+)/Ag(+) sensor histidine kinase	-
AM388_RS17020	3372033..3372716	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
AM388_RS17025	3372889..3374274	+	1386	WP_032412712.1	efflux transporter outer membrane subunit	-
AM388_RS17030	3374292..3374636	+	345	WP_021441118.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19796.62 Da
• Isoelectric Point: 5.2614

>T104496 WP_068815439.1 NZ_CP028953:c3370230-3369697 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDISGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T104496 NZ_CP028953:c3370230-3369697 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCTCTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT104496 WP_002887278.1 NZ_CP028953:c3370507-3370241 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT104496 NZ_CP028953:c3370507-3370241 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1