Detailed information of TA system TA104476

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 2575472..2576139
• Replicon: chromosome
• Accession: NZ_CP028950
• Organism: Enterobacter cloacae complex sp. FDA-CDC-AR_0164

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: AM410_RS12690
• Protein ID: WP_108647911.1
• Coordinates: 2575472..2575801 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AM410_RS12695
• Protein ID: WP_108647913.1
• Coordinates: 2575822..2576139 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM410_RS12650	2570806..2571336	+	531	WP_080346980.1	sigma-70 family RNA polymerase sigma factor	-
AM410_RS12655	2571333..2572121	+	789	WP_047356646.1	membrane protein	-
AM410_RS12660	2572113..2572670	-	558	WP_025203421.1	YceI family protein	-
AM410_RS12665	2572739..2573083	-	345	WP_020883895.1	hypothetical protein	-
AM410_RS12670	2573171..2573527	-	357	WP_014171277.1	hypothetical protein	-
AM410_RS12675	2573748..2574188	-	441	WP_073396531.1	hypothetical protein	-
AM410_RS12680	2574439..2574903	-	465	Protein_2396	hypothetical protein	-
AM410_RS12685	2574906..2575202	-	297	WP_108647910.1	hypothetical protein	-
AM410_RS12690	2575472..2575801	-	330	WP_108647911.1	TA system toxin CbtA family protein	Toxin
AM410_RS12695	2575822..2576139	-	318	WP_108647913.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM410_RS12700	2576158..2576379	-	222	WP_000691992.1	DUF987 domain-containing protein	-
AM410_RS12705	2576388..2576870	-	483	WP_040083479.1	DNA repair protein RadC	-
AM410_RS12710	2576879..2577337	-	459	WP_000211836.1	antirestriction protein	-
AM410_RS12715	2577439..2577662	-	224	Protein_2403	DUF905 family protein	-
AM410_RS12720	2577960..2580125	-	2166	WP_108648586.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2574586..2599794	25208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12406.44 Da
• Isoelectric Point: 9.5496

>T104476 WP_108647911.1 NZ_CP028950:c2575801-2575472 [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFSDETVIKEHIDAGITLTDAVNFLVDKYELVRFDRKGF
SWQEQTPYLSVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T104476 NZ_CP028950:c2575801-2575472 [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
ATGAAAACTCTACCGGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTTCTTAC
ACGGCTACTAGAAAAACACTATGGTCTGACCCTGAACGATACGCCATTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCTGGTATCACCTTGACTGATGCAGTCAACTTTCTGGTGGATAAGTACGAACTAGTGCGTTTTGATCGGAAAGGATTT
TCGTGGCAAGAGCAGACCCCGTATCTTTCCGTAGTGGATATTTTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
CGTGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.49 Da
• Isoelectric Point: 6.6247

>AT104476 WP_108647913.1 NZ_CP028950:c2576139-2575822 [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
MSNITWGLQRDITPRLGARLVQEGNRLHYLAERANITDKFSDAECLKLDVAFPHFISQMESMLTTGEMNPRHAHCVTLNH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT104476 NZ_CP028950:c2576139-2575822 [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
ATGAGTAACATCACATGGGGCCTGCAGCGGGACATTACGCCACGCCTGGGAGCCCGTCTGGTGCAGGAGGGGAACCGGCT
GCACTATCTGGCTGAGCGGGCAAATATCACCGATAAGTTTAGTGATGCCGAGTGTCTGAAGTTGGATGTCGCATTCCCGC
ATTTTATCAGTCAGATGGAATCAATGCTGACCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGAACCAC
AATGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATATATCGCCATTTATCCCACTCAACGCTAA